{"id":1987,"date":"2025-02-21T12:32:43","date_gmt":"2025-02-21T11:32:43","guid":{"rendered":"https:\/\/sita-slovensko.com\/mereni-intenzity-fluorescence\/"},"modified":"2025-06-05T07:16:48","modified_gmt":"2025-06-05T05:16:48","slug":"mereni-intenzity-fluorescence","status":"publish","type":"post","link":"https:\/\/sita-slovensko.com\/cs\/mereni-intenzity-fluorescence\/","title":{"rendered":"M\u011b\u0159en\u00ed intenzity fluorescence"},"content":{"rendered":"\n
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Metoda m\u011b\u0159en\u00ed fluorescence vyu\u017e\u00edv\u00e1 vlastnost organick\u00fdch l\u00e1tek<\/strong> fluoreskovat. UV sv\u011btlo<\/strong> je zam\u011b\u0159eno na povrch, co\u017e zp\u016fsobuje, \u017ee organick\u00e9 l\u00e1tky za\u010dnou fluoreskovat. Se zvy\u0161uj\u00edc\u00ed se tlou\u0161\u0165kou organick\u00e9 vrstvy se zvy\u0161uje i intenzita fluorescence. Intenzita se m\u011b\u0159\u00ed v RFU<\/strong>: Relativn\u00ed fluorescen\u010dn\u00ed jednotky. <\/p>\n<\/div>\n<\/div>\n\n

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Fluorescence <\/h2>\n\n\n\n

Fluorescence je speci\u00e1ln\u00ed forma luminiscence. Je excitov\u00e1na ultrafialov\u00fdm z\u00e1\u0159en\u00edm. Elektrony ve fluorescen\u010dn\u00edch molekul\u00e1ch absorbuj\u00ed fotony a p\u0159ech\u00e1zej\u00ed na vy\u0161\u0161\u00ed energetickou hladinu. Tento excitovan\u00fd stav je energeticky nestabiln\u00ed. Elektrony se rychle vracej\u00ed do z\u00e1kladn\u00edho stavu a p\u0159i tom uvol\u0148uj\u00ed absorbovanou energii. V\u00fdsledkem je emise fluorescen\u010dn\u00edho sv\u011btla. Vzhledem k tomu, \u017ee \u010d\u00e1st energie se p\u0159em\u011bn\u00ed na teplo, vyza\u0159ovan\u00e9 z\u00e1\u0159en\u00ed je m\u00e9n\u011b energetick\u00e9 a m\u00e1 del\u0161\u00ed vlnovou d\u00e9lku. <\/p>\n<\/div>\n\n\n\n

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M\u011b\u0159en\u00ed intenzity fluorescence<\/h2>\n\n\n\n

Organick\u00e9 l\u00e1tky jako oleje, tuky nebo emulze jsou excitov\u00e1ny k fluorescenci UV zdrojem sv\u011btla. Fotodioda m\u011b\u0159\u00ed intenzitu z\u00e1\u0159en\u00ed emitovan\u00e9ho fluorescenc\u00ed p\u0159i ur\u010den\u00e9 vlnov\u00e9 d\u00e9lce v modr\u00e9m sv\u011bteln\u00e9m rozsahu. Intenzita se m\u011b\u0159\u00ed v RFU (Relativn\u00ed fluorescen\u010dn\u00ed jednotky). \u010c\u00edm vy\u0161\u0161\u00ed je hodnota RFU, t\u00edm vy\u0161\u0161\u00ed je intenzita fluorescen\u010dn\u00edho sv\u011btla. <\/p>\n\n\n\n

D\u00edky kalibraci se standardy fluorescence SITA jsou nam\u011b\u0159en\u00e9 hodnoty RFU srovnateln\u00e9.<\/p>\n\n\n\n

Fluorescen\u010dn\u00ed m\u011b\u0159ic\u00ed za\u0159\u00edzen\u00ed SITA pracuj\u00ed na principu konfok\u00e1ln\u00edho m\u011b\u0159en\u00ed. Excita\u010dn\u00ed sv\u011btlo a sv\u011btlo emitovan\u00e9 fluorescenc\u00ed sleduj\u00ed stejnou paraleln\u00ed dr\u00e1hu. Fluorescence m\u011b\u0159en\u00e1 v kapalin\u011b nebo na povr\u0161\u00edch je kumulativn\u00edm parametrem v\u0161ech p\u0159\u00edtomn\u00fdch fluorescen\u010dn\u00edch l\u00e1tek. Pokud jsou p\u0159\u00edtomny sm\u011bsi l\u00e1tek, nam\u011b\u0159en\u00e1 fluorescence nedok\u00e1\u017ee ur\u010dit, kter\u00e9 l\u00e1tky jsou p\u0159\u00edtomn\u00e9 ani jejich jednotliv\u00e9 koncentrace. <\/p>\n<\/div>\n<\/div>\n\n

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Fluorescence kontaminant\u016f<\/h2>\n\n\n\n

V\u00fdrobn\u00ed pom\u016fcky jako oleje, tuky, chladic\u00ed kapaliny a separa\u010dn\u00ed \u010dinidla jsou technick\u00e9 kapaliny. Jejich fluorescence je zp\u016fsobena p\u0159edev\u0161\u00edm nenasycen\u00fdmi uhlovod\u00edkov\u00fdmi strukturami a p\u0159idan\u00fdmi aditivy s aromatick\u00fdmi kruhov\u00fdmi syst\u00e9my. Kyseliny karboxylov\u00e9, jejich estery a alifatick\u00e9 ketony tak\u00e9 fluoreskuj\u00ed. <\/p>\n\n\n\n

M\u011b\u0159en\u00ed fluorescence dok\u00e1\u017ee zjistit i nejmen\u0161\u00ed mno\u017estv\u00ed fluorescen\u010dn\u00edch l\u00e1tek. Dokonce i otisky prst\u016f, kter\u00e9 obsahuj\u00ed m\u00e9n\u011b ne\u017e 2 % ko\u017en\u00edch tuk\u016f, mohou b\u00fdt detekov\u00e1ny. Typick\u00e9 rozsahy detekce pro v\u00fdrobn\u00ed pom\u016fcky jsou 10 mg\/m\u00b2 \u2026 10 g\/m\u00b2 (1 \u03bcg\/cm\u00b2 \u2026 1 mg\/cm\u00b2). <\/p>\n\n\n\n

V\u00fdjimkami jsou l\u00e1tky, kter\u00e9 nefluoreskuj\u00ed p\u0159i UV excitaci, jako jsou silikonov\u00e9 oleje, nasycen\u00e9 organick\u00e9 slou\u010deniny, kr\u00e1tko\u0159et\u011bzcov\u00e9 nenasycen\u00e9 uhlovod\u00edky, kovy a jejich oxidy. Zda je mo\u017en\u00e9 detekovat kontaminaci v mno\u017estv\u00ed relevantn\u00edm pro proces (lepen\u00ed, sva\u0159ov\u00e1n\u00ed apod.), lze otestovat jednoduch\u00fdm fluorescen\u010dn\u00edm testem. <\/p>\n\n\n\n

P\u0159id\u00e1n\u00edm fluorescen\u010dn\u00edch pigment\u016f nebo barviv jako fluorescen\u010dn\u00edch marker\u016f mohou b\u00fdt detekov\u00e1ny i slab\u011b nebo nefluoreskuj\u00edc\u00ed l\u00e1tky. Fluorescen\u010dn\u00ed markery se pou\u017e\u00edvaj\u00ed ke sn\u00ed\u017een\u00ed detek\u010dn\u00edho limitu a zaznamen\u00e1n\u00ed tlou\u0161t\u011bk vrstev pod 10 mg\/m\u00b2 (1 \u03bcg\/cm\u00b2). <\/p>\n<\/div>\n<\/div>\n\n

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Fluorescence substr\u00e1t\u016f<\/h2>\n\n\n\n

Kovov\u00e9 a keramick\u00e9 povrchy nefluoreskuj\u00ed. Sklen\u011bn\u00e9 povrchy mohou fluoreskovat v d\u016fsledku ne\u010distot v amorfn\u00ed struktu\u0159e. Jin\u00e9 materi\u00e1ly jako pap\u00edr, textilie a plasty maj\u00ed tendenci v\u00edce fluoreskovat d\u00edky sv\u00e9 slo\u017eit\u00e9 struktu\u0159e organick\u00fdch molekul. <\/p>\n<\/div>\n<\/div>\n\n

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Fluorescence substr\u00e1t\u016f<\/h2>\n\n\n\n

Kovov\u00e9 a keramick\u00e9 povrchy nefluoreskuj\u00ed. Sklen\u011bn\u00e9 povrchy mohou fluoreskovat v d\u016fsledku ne\u010distot v amorfn\u00ed struktu\u0159e. Jin\u00e9 materi\u00e1ly jako pap\u00edr, textilie a plasty maj\u00ed tendenci v\u00edce fluoreskovat d\u00edky sv\u00e9 slo\u017eit\u00e9 struktu\u0159e organick\u00fdch molekul. <\/p>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

Metoda m\u011b\u0159en\u00ed fluorescence vyu\u017e\u00edv\u00e1 vlastnost organick\u00fdch l\u00e1tek fluoreskovat. UV sv\u011btlo je zam\u011b\u0159eno na povrch, co\u017e zp\u016fsobuje, \u017ee organick\u00e9 l\u00e1tky za\u010dnou fluoreskovat. <\/p>\n","protected":false},"author":1,"featured_media":1712,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[34],"tags":[],"class_list":["post-1987","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-informace"],"acf":[],"_links":{"self":[{"href":"https:\/\/sita-slovensko.com\/cs\/wp-json\/wp\/v2\/posts\/1987","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sita-slovensko.com\/cs\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/sita-slovensko.com\/cs\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/sita-slovensko.com\/cs\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/sita-slovensko.com\/cs\/wp-json\/wp\/v2\/comments?post=1987"}],"version-history":[{"count":0,"href":"https:\/\/sita-slovensko.com\/cs\/wp-json\/wp\/v2\/posts\/1987\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/sita-slovensko.com\/cs\/wp-json\/wp\/v2\/media\/1712"}],"wp:attachment":[{"href":"https:\/\/sita-slovensko.com\/cs\/wp-json\/wp\/v2\/media?parent=1987"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/sita-slovensko.com\/cs\/wp-json\/wp\/v2\/categories?post=1987"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/sita-slovensko.com\/cs\/wp-json\/wp\/v2\/tags?post=1987"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}